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The aim of this moduleis to provide to students the insights into the application of animal tissue culture. It involves correlation with practical course where possible and study tour to ensure full understanding of the techniques used in tissue culture and help the students to understand how to develop micro-environment and which factors will affect tissue culture, media formulations, cell lines and different applications of tissue culture
COURSE CONTENTS
BIO2265-Animal and Plant Tissue Culture
1.Introduction on tissue culture
Definition and Historical Background
Advantages and limitations.
Characteristic features of Cell culture
Culture medium formulation
2.Tissue Culture Methodology.
Equipment used in tissue culture
Types of cultures
3.Cell lines characterization
Cross contamination hazards.
Characteristics of cells in culture.
Growth studies: cell proliferation, cell cycle, mitosis in growing cells.
4.Applications of tissue culture:
Tissue culture as a screening system;
Cytotoxicity and diagnostic tests.
Development and preparation of vaccines against infecting organisms.