BTC3161 Animal and Plant Tissue Culture

The aim of this course is to provide to students the insights into the application of animal  and plant tissue culture. It involves correlation with practical course to ensure full understanding of the techniques used in tissue culture and help the students to understand how to develop micro-environment and which factors will affect tissue culture, media formulations, cell lines and different applications of tissue culture.

Indicative Content: 

Plant tissue culture

1.Introduction ( History).

2. Preparation  and composition of nutrient media.

3.Care of Plant material .

4.Isolation, inoculation and subculturing. 

5.Transfer from nutrient medium to soil .

6.Embryon culture .

7.Production of disease free plants.

8.Vegetative propagation.

9.Somaclonal  variation.

Animal Tissue Culture

1. Origin and principles of cell culture
2. In vitro animal tissue culture
3. Culture medium formulation, Mammalian cell culture; Stem cell culture, Qualitative characteristics of cell cultures, cell counting and analysis, Primary cell culture techniques, Subculture and Development of immortalized cell line, Detection of microbial contaminants
4. Advantages and limitations of animal tissue culture
5. Cryopreservation and Cell banking. Applications of tissue culture